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pyromark vacuum prep tool  (Qiagen)


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    Structured Review

    Qiagen pyromark vacuum prep tool
    Pyromark Vacuum Prep Tool, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pyromark+vacuum+prep+tool/pyromark+vacuum+prep+tool/pmc11418189-92-34-38
    Average 90 stars, based on 1 article reviews
    pyromark vacuum prep tool - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Manipulation of a social signal affects DNA methylation of a stress-related gene in a free-living bird
    Article Snippet: .. PCR product was bound to Streptavidin Sepharose HP (GE Healthcare Life Sciences), after which the immobilized PCR products were purified, washed, denatured with a 0.2 μmol l −1 NaOH solution, and rewashed using the Pyromark Vacuum Prep Tool (Qiagen), as per the manufacturer's protocol. .. Next, 0.5 μmol l −1 of sequencing primer was annealed to the purified single-stranded PCR products and 10 μl of the PCR products was pyrosequenced on the PSQ96 HS System (Qiagen) following the manufacturer's instructions.

    Article Title: DNA demethylation and tri-methylation of H3K4 at the TACSTD2 promoter are complementary players for TROP2 regulation in colorectal cancer cells
    Article Snippet: .. The PCR product was prepared for pyrosequencing using the PyroMark vacuum prep tool (Qiagen). .. Using the corresponding sequencing primers for our four primer pairs, the samples were analysed by PyroMark Q24 (Qiagen).

    Article Title: X-Chromosome Inactivation is a Biomarker of Clinical Severity in Female Carriers of RPGR -Associated X-Linked Retinitis Pigmentosa
    Article Snippet: DNA sequences containing SNPs of interest were amplified from 37.5 ng genomic DNA with the PyroMark PCR Kit (Qiagen), using a primer with an M13 tail and a universal biotinylated M13 primer. .. PCR product was captured on a PyroMark Vacuum Prep Tool (Qiagen) by aspiration and washed in 70% ethanol, Pyromark Denaturation Solution (Qiagen), and PyroMark Wash Buffer (Qiagen). .. PCR product was released into 15 uL of 0.3 uM sequencing primer diluted in PyroMark Annealing Buffer (Qiagen), heated at 70°C for 15 minutes, and cooled at room temperature for 10 minutes before running on a PSQ hs 96A Instrument (Qiagen).

    Article Title: Molecular evaluation of BRAF gene mutation in thyroid tumors: Significant association with papillary tumors and extra thyroidal extension indicating its role as a biomarker of aggressive disease.
    Article Snippet: Background: Somatic mutation of the BRAF gene is one to be the most commonly known genetic change in thyroid tumors especially papillary thyroid cancers.. The T1799A activating point mutation is detected in>98% of the thyroid tumors, and result in substitution of amino acid valine at position 600 to glutamic acid.. Method: In this study, we evaluated BRAF mutation in 95 Indian thyroid tumors by pyrosequencing assay.

    Purification:

    Article Title: Manipulation of a social signal affects DNA methylation of a stress-related gene in a free-living bird
    Article Snippet: .. PCR product was bound to Streptavidin Sepharose HP (GE Healthcare Life Sciences), after which the immobilized PCR products were purified, washed, denatured with a 0.2 μmol l −1 NaOH solution, and rewashed using the Pyromark Vacuum Prep Tool (Qiagen), as per the manufacturer's protocol. .. Next, 0.5 μmol l −1 of sequencing primer was annealed to the purified single-stranded PCR products and 10 μl of the PCR products was pyrosequenced on the PSQ96 HS System (Qiagen) following the manufacturer's instructions.

    Sequencing:

    Article Title: Molecular evaluation of BRAF gene mutation in thyroid tumors: Significant association with papillary tumors and extra thyroidal extension indicating its role as a biomarker of aggressive disease.
    Article Snippet: Background: Somatic mutation of the BRAF gene is one to be the most commonly known genetic change in thyroid tumors especially papillary thyroid cancers.. The T1799A activating point mutation is detected in>98% of the thyroid tumors, and result in substitution of amino acid valine at position 600 to glutamic acid.. Method: In this study, we evaluated BRAF mutation in 95 Indian thyroid tumors by pyrosequencing assay.

    other:

    Article Title: Strong association between glucocerebrosidase mutations and Parkinson's disease in Sweden
    Article Snippet: After PCR, the biotinylated PCR product was immobilized to streptavidin-coated beads (GE Healthcare, Buckinghamshire, United Kingdom), mixed for 10 minutes at room temperature and captured onto filter probes (PyroMark Vacuum Prep Tool, Qiagen, Venlo, the Netherlands).



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